This in-person, hands-on workshop is appropriate for students, staff, fellows and faculty working with high throughput transcriptomic data. This is the first part of a two-workshop series. Attendance at this session, or instructor permission, is required before taking RNA-Seq Analysis Part2. In this session you will learn the basics of high throughput RNA sequencing, including how to check the quality of your RNA-Seq reads, how to determine if reads contain adaptor sequences, and if so, how to identify and remove the adaptor sequence from raw RNA-Seq data. We will discuss all the basic points in detail. At the end of the workshop, you will learn how to connect to Rivanna and run an RNA-Seq pipeline (mapping and gene quantitation) on Rivanna or on your own system.

Prerequisites:

• No previous experience with the Unix operating system, Rivanna, programming, or genomics is required.
• Attendees must bring a laptop to the session in order to connect to Rivanna compute system.